Using the AD12-ICT rapid-format test to detect Wuchereria bancrofti circulating antigens in comparison to Og4C3-ELISA and nucleopore membrane filtration and microscopy techniques.
Identifieur interne : 003D58 ( Main/Exploration ); précédent : 003D57; suivant : 003D59Using the AD12-ICT rapid-format test to detect Wuchereria bancrofti circulating antigens in comparison to Og4C3-ELISA and nucleopore membrane filtration and microscopy techniques.
Auteurs : Amal Abdul-Rasheed El-Moamly [Égypte] ; Mohamed Aly El-Sweify ; Mohamad Abdul HafezSource :
- Parasitology research [ 1432-1955 ] ; 2012.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Antigènes d'helminthe (sang), Arabie saoudite (épidémiologie), Femelle, Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Filtration (), Filtration (instrumentation), Humains, Immunochromatographie (), Jeune adulte, Membrane artificielle, Microscopie (), Mâle, Reproductibilité des résultats, Sensibilité et spécificité, Test ELISA (), Wuchereria bancrofti (immunologie), Wuchereria bancrofti (isolement et purification), Études cas-témoins.
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Wuchereria bancrofti.
- isolement et purification : Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Antigènes d'helminthe, Filtration.
- épidémiologie : Arabie saoudite.
- Adulte, Adulte d'âge moyen, Animaux, Femelle, Filtration, Humains, Immunochromatographie, Jeune adulte, Membrane artificielle, Microscopie, Mâle, Reproductibilité des résultats, Sensibilité et spécificité, Test ELISA, Études cas-témoins.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Adult, Animals, Antigens, Helminth (blood), Case-Control Studies, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay (methods), Female, Filtration (instrumentation), Filtration (methods), Humans, Immunochromatography (methods), Male, Membranes, Artificial, Microscopy (methods), Middle Aged, Reproducibility of Results, Saudi Arabia (epidemiology), Sensitivity and Specificity, Wuchereria bancrofti (immunology), Wuchereria bancrofti (isolation & purification), Young Adult.
- MESH :
- chemical , blood : Antigens, Helminth.
- geographic , epidemiology : Saudi Arabia.
- diagnosis : Elephantiasis, Filarial.
- immunology : Wuchereria bancrofti.
- instrumentation : Filtration.
- isolation & purification : Wuchereria bancrofti.
- methods : Enzyme-Linked Immunosorbent Assay, Filtration, Immunochromatography, Microscopy.
- parasitology : Elephantiasis, Filarial.
- Adult, Animals, Case-Control Studies, Female, Humans, Male, Membranes, Artificial, Middle Aged, Reproducibility of Results, Sensitivity and Specificity, Young Adult.
Abstract
Lymphatic filariasis (LF) continues to be a major source of permanent disability and an impediment to socio-economic development in 73 countries where more than 1 billion people are at risk and over 120 millions are infected. The global drive to eliminate LF necessitates an increasing demand for valid, reliable and rapid diagnostic tests. This study aimed to assess the performance of the AD12 rapid format immunochromatographic test (ICT) to detect Wuchereria bancrofti circulating antigens, against the combined gold standard: TropBio Og4C3-ELISA (enzyme-linked immunosorbent assay) which detects circulating filarial antigen (CFA) and the nucleopore membrane filtration and microscopic examination. This prospective case-control study involved 647 asymptomatic migrant workers from filariasis-endemic countries. Of these specimens, 32 were positive for microfilaremia using the membrane filtration and microscopy, 142 positive by ELISA (of which 32 had microfilaremia), and 128 positive by the ICT (of which 31 had microfilaremia). The performance of the ICT was calculated against 32 true-positive and 90 true-negative cases. For the detection of CFA, the ICT had a sensitivity of 97% (95% confidence interval [CI] 91-103), specificity 100% (95% CI 100-100), Positive Predictive Value (PPV) 100% (95% CI 100-100), Negative Predictive Value (NPV) 99% (95% CI 97-101); and the total accuracy of the test was 99% (95% CI 98-101). The agreement between ICT and ELISA in detecting W. bancrofti antigens was excellent (kappa = 0.934; p = 0.000). In conclusion, the AD12-ICT test for the detection of W. bancrofti-CFA was sensitive and specific and comparable to the performance of ELISA. The ICT would be a useful additional test to facilitate the proposed strategies for control and elimination of LF. Because it is rapid, simple to perform, and does not require the use of special equipment, the ICT may be most appropriate in screening programs and in monitoring the possible risk of introducing the disease to the non-endemic countries.
DOI: 10.1007/s00436-012-2870-5
PubMed: 22392137
Affiliations:
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Le document en format XML
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adulte</term><term>Membrane artificielle</term><term>Microscopie ()</term><term>Mâle</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Test ELISA ()</term><term>Wuchereria bancrofti (immunologie)</term><term>Wuchereria bancrofti (isolement et purification)</term><term>Études cas-témoins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Saudi Arabia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria 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saoudite</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Case-Control Studies</term><term>Female</term><term>Humans</term><term>Male</term><term>Membranes, Artificial</term><term>Middle Aged</term><term>Reproducibility of Results</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Femelle</term><term>Filtration</term><term>Humains</term><term>Immunochromatographie</term><term>Jeune adulte</term><term>Membrane artificielle</term><term>Microscopie</term><term>Mâle</term><term>Reproductibilité des résultats</term><term>Sensibilité et spécificité</term><term>Test ELISA</term><term>Études cas-témoins</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis (LF) continues to be a major source of permanent disability and an impediment to socio-economic development in 73 countries where more than 1 billion people are at risk and over 120 millions are infected. The global drive to eliminate LF necessitates an increasing demand for valid, reliable and rapid diagnostic tests. This study aimed to assess the performance of the AD12 rapid format immunochromatographic test (ICT) to detect Wuchereria bancrofti circulating antigens, against the combined gold standard: TropBio Og4C3-ELISA (enzyme-linked immunosorbent assay) which detects circulating filarial antigen (CFA) and the nucleopore membrane filtration and microscopic examination. This prospective case-control study involved 647 asymptomatic migrant workers from filariasis-endemic countries. Of these specimens, 32 were positive for microfilaremia using the membrane filtration and microscopy, 142 positive by ELISA (of which 32 had microfilaremia), and 128 positive by the ICT (of which 31 had microfilaremia). The performance of the ICT was calculated against 32 true-positive and 90 true-negative cases. For the detection of CFA, the ICT had a sensitivity of 97% (95% confidence interval [CI] 91-103), specificity 100% (95% CI 100-100), Positive Predictive Value (PPV) 100% (95% CI 100-100), Negative Predictive Value (NPV) 99% (95% CI 97-101); and the total accuracy of the test was 99% (95% CI 98-101). The agreement between ICT and ELISA in detecting W. bancrofti antigens was excellent (kappa = 0.934; p = 0.000). In conclusion, the AD12-ICT test for the detection of W. bancrofti-CFA was sensitive and specific and comparable to the performance of ELISA. The ICT would be a useful additional test to facilitate the proposed strategies for control and elimination of LF. Because it is rapid, simple to perform, and does not require the use of special equipment, the ICT may be most appropriate in screening programs and in monitoring the possible risk of introducing the disease to the non-endemic countries.</div></front></TEI><affiliations><list><country><li>Égypte</li></country></list><tree><noCountry><name sortKey="El Sweify, Mohamed Aly" sort="El Sweify, Mohamed Aly" uniqKey="El Sweify M" first="Mohamed Aly" last="El-Sweify">Mohamed Aly El-Sweify</name><name sortKey="Hafez, Mohamad Abdul" sort="Hafez, Mohamad Abdul" uniqKey="Hafez M" first="Mohamad Abdul" last="Hafez">Mohamad Abdul Hafez</name></noCountry><country name="Égypte"><noRegion><name sortKey="El Moamly, Amal Abdul Rasheed" sort="El Moamly, Amal Abdul Rasheed" uniqKey="El Moamly A" first="Amal Abdul-Rasheed" last="El-Moamly">Amal Abdul-Rasheed El-Moamly</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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